Inverted fluorescence microscopes
Fluorescence microscopy is the most demanding technique in biology, biomedical sciences, and materials science, capable of studying organic and inorganic samples through primary fluorescence (auto-fluorescence) or secondary fluorescence using fluorochrome staining and labeling. On an inverted stand, it lets researchers observe fluorescently labeled live cell cultures directly in their flask or dish, without disturbing them. Optika’s inverted fluorescence range spans the IM-300 series in HBO and LED variants, the research-grade IM-5FLD with the widest fluorochrome coverage in this range, and the expandable IM-7 platform, upgradable with an HBO fluorescence package for research, clinical, and pharmaceutical diagnostic use.
- Type: Inverted HBO fluorescence, trinocular
- Magnification: Up to 400x
- Optics: IOS LWD W-PLAN PH
- Illumination: HBO 100W, X-LED8 transmitted
- Head: Trinocular, 45 degree inclined
€7.544,00 excl. VAT
- Type: Inverted HBO fluorescence, trinocular
- Magnification: Up to 400x
- Optics: IOS LWD U-PLAN F
- Illumination: HBO 100W, X-LED8 transmitted
- Head: Trinocular, 45 degree inclined
€10.768,00 excl. VAT
- Type: Inverted LED fluorescence, trinocular
- Magnification: Up to 400x
- Optics: IOS LWD W-PLAN PH
- Illumination: LED Fluorescence Cube, X-LED8 transmitted
- Head: Trinocular, 45 degree inclined
€7.530,00 excl. VAT
Optika IM-300LD4 Inverted LED fluorescence microscope, 400x, IOS LWD PLAN F, 4 empty filterset slots
- Type: Inverted LED fluorescence, trinocular
- Magnification: Up to 400x
- Optics: IOS LWD U-PLAN F, configurable fluorescence
- Illumination: LED Fluorescence Cube, X-LED8 transmitted
- Head: Trinocular, 45 degree inclined
€8.234,00 excl. VAT
- Type: Inverted LED fluorescence, digital
- Magnification: Up to 400x
- Optics: IOS LWD U-PLAN F, configurable fluorescence
- Illumination: LED Fluorescence Cube, X-LED8 transmitted
- Head: Trinocular with 6.3 MP camera
€10.893,00 excl. VAT
- Type: Inverted LED fluorescence, trinocular
- Magnification: Up to 600x
- Optics: IOS LWD, configurable objective platform
- Illumination: X-LED8 transmitted, B/G/UV fluorescence LEDs
- Head: Trinocular, 45 degree inclined
€10.832,00 excl. VAT
Comparing Optika Inverted Fluorescence Models
| Model | Light source | Filter configuration | Objectives | Notable feature |
|---|---|---|---|---|
| IM-300F | HBO 100 W mercury | 2 positions, blue and green included | IOS LWD W-PLAN PH | Routine brightfield, phase contrast, and fluorescence |
| IM-300FL4 | HBO 100 W mercury | 4 positions, blue and green included, expandable | Semi-Apo IOS LWD U-PLAN F | Enhanced contrast, room to add filter sets |
| IM-300LD2 | LED, 2 cubes | 2 positions, blue and green included | IOS LWD W-PLAN PH | LED convenience, brightfield and phase contrast too |
| IM-300LD4 | LED, 4 positions | Configurable, matched to your fluorochromes | IOS U-PLAN F | Modular LED fluorescence platform |
| IM-300LD4D | LED, 4 positions | Configurable, matched to your fluorochromes | IOS U-PLAN F | Same as IM-300LD4, with built-in camera and 4K touchscreen |
| IM-5FLD | LED, 4 positions, 5 W | Blue, green, and UV included, widest fluorochrome range | Freely configurable, 24 mm field number | |
| IM-7 | Optional HBO package | Upgradable as research needs grow | Configurable, sold separately | Flagship, expandable research and clinical platform |
HBO or LED: Choosing an Illumination Source
Optika offers both traditional HBO mercury lamp and LED fluorescence sources across the IM-300 series. HBO models (IM-300F, IM-300FL4) give a brighter, broader-spectrum source, the most widely used method worldwide for fluorescence research, at the cost of a limited bulb lifetime and warm-up time before use. LED models (IM-300LD2, IM-300LD4, IM-300LD4D, IM-5FLD) eliminate warm-up and cool-down delays entirely and remove the inconvenience of lamp replacement and centering, at a running cost and maintenance advantage that suits routine and high-throughput fluorescence work.
Choosing the Right Model
IM-300F is Optika’s routine HBO fluorescence model, combining transmitted brightfield, phase contrast, and fluorescence with IOS LWD W-PLAN PH objectives. Its 2-position blue and green filter set visualizes common fluorochromes such as Acridine Yellow, Acridine Orange, Auramine, FITC, and GFP with the blue filter, and Rhodamine, Texas Red, and TRITC with the green filter.
IM-300FL4 moves to Semi-Apochromatic IOS LWD U-PLAN F objectives for enhanced sample visibility and contrast, with a 4-position filter holder that ships with blue and green filter sets standard and accepts additional filter sets for future upgrades, suited to laboratories that expect their fluorochrome needs to grow.
IM-300LD2 is the LED equivalent of IM-300F, pairing the same IOS LWD W-PLAN PH objectives (brightfield, phase contrast, and fluorescence) with 2 LED cubes covering the same blue and green fluorochrome range, for routine work without lamp maintenance.
IM-300LD4 and IM-300LD4D use IOS U-PLAN F fluorescence-optimized objectives with a 4-position LED filter platform for visualizing most fluorochromes, letting the laboratory match filter cubes to its specific research needs. IM-300LD4D adds a built-in Intel micro PC, 15.6 inch 4K touchscreen, 6 MP high-sensitivity camera, and Optika ProView software for documenting fluorescence directly on screen.
IM-5FLD is the research-grade option, combining brightfield, phase contrast, and darkfield with a 4-position LED epi-fluorescence attachment powered by 5 W LEDs. It covers the widest fluorochrome range in the inverted fluorescence line, with blue, green, and UV filters visualizing everything from common fluorochromes like FITC and GFP to specialized UV-excited dyes such as DAPI, Hoechst, and Fura-2, on a freely configurable objective platform with a 24 mm field number.
IM-7 does not include fluorescence as standard, but is tailored for research, clinical, and pharmaceutical diagnostic applications and upgrades to HBO mercury lamp fluorescence through its optional fluorescence package, making it the choice for research groups that need fluorescence as one part of a larger, expandable imaging platform.
Related Microscope Categories
If your sample needs a simpler setup, see inverted brightfield microscopes for routine live cell observation, or inverted phase contrast microscopes for unstained internal cell structure. For opaque samples viewed from below, see inverted metallurgical microscopes.
If your sample is already mounted on a standard glass slide, see our upright fluorescence microscopes. For the full inverted range overview, see inverted microscopes.