Optika IM-300F Inverted HBO fluorescence/phase contrast microscope, 400x, IOS LWD PLAN, B&G filter set

€7.544,00 excl. VAT

Estimated delivery time: 5-7 weeks

The Optika IM-300F is an inverted trinocular microscope for brightfield, phase contrast, and HBO fluorescence observation of live cell cultures. It carries IOS LWD W-PLAN PH objectives at 10x, 20x, and 40x, and the condenser extends its working distance up to 135 mm for tall culture vessels. The fluorescence attachment ships with blue and green filter sets installed on a 3-position holder, powered by a 100 W HBO mercury lamp. It suits life science laboratories that need traditional mercury lamp fluorescence alongside inverted brightfield and phase contrast.

Inverted HBO Fluorescence Microscope with Blue and Green Filter Sets Included

The Optika IM-300F brings traditional HBO mercury lamp fluorescence to an inverted stand for cell culture observation, with blue and green filter sets included as standard. The condenser extends its working distance up to 135 mm, and a phase contrast slider adds brightfield and phase contrast on the same stand.

HBO 100 W high pressure mercury lamp for fluorescence excitation
Blue and green filter sets included, 3-position holder
Condenser working distance extendable up to 135 mm for tall culture vessels
IOS LWD W-PLAN PH objectives at 10x, 20x, and 40x for brightfield and phase contrast
Suited to inverted cell culture fluorescence workflows

Optika IM-300F Inverted Trinocular HBO Fluorescence Microscope

The Optika IM-300F is an inverted trinocular microscope from the IM-300 series, built around a 100 W HBO high pressure mercury lamp for cell culture fluorescence work.

IOS LWD W-PLAN PH objectives cover brightfield and phase contrast observation, with an extendable working distance condenser for tall culture vessels.

HBO Mercury Lamp Fluorescence with Included Filter Sets

The fluorescence attachment ships with blue and green filter sets installed on a 3-position holder, powered by a 100 W HBO high pressure mercury bulb with a 300 hour lifetime.

IOS LWD W-PLAN PH Objectives

The objective set covers 10x, 20x, and 40x, infinity corrected and anti-fungus treated with a 45 mm parfocal distance, giving 100x to 400x total magnification.

Extendable Long Working Distance Condenser

The Abbe condenser, N.A. 0.30, has a standard 72 mm working distance that extends up to 135 mm, with a phase contrast slider (BF, 4x/10x, 20x/40x) and a color filter slider.

X-LED8 Transmitted Illumination

The X-LED8 light source covers the transmitted brightfield and phase contrast light path at 8 W with manual brightness control.

Ideal Applications

The IM-300F suits laboratories that need inverted HBO fluorescence alongside brightfield and phase contrast.

Live cell culture fluorescence imaging
Immunofluorescence with blue and green fluorochromes
Phase contrast observation of unstained cell cultures
Research applications needing tall culture vessel compatibility
Cell biology laboratories transitioning to fluorescence work

Choose the IM-300F when:

You need traditional HBO mercury lamp fluorescence included as standard
You need an extendable working distance condenser for tall vessels
You need brightfield, phase contrast, and fluorescence on one inverted stand
You want blue and green filter coverage without ordering separately

Optika Value Proposition

The Optika IM-300F combines IOS LWD W-PLAN PH optics with HBO mercury lamp fluorescence included as standard, on an inverted trinocular stand with an extendable long working distance condenser.

Technical specifications – IM-300F

Specification	IM-300F
Type	Inverted, aluminum die-cast
Head	Trinocular, Siedentopf, split ratio 100/0 – 0/100, 45 degree inclination
Eyepieces	PL 10x, field number 22 mm, high eyepoint, retractable protections
Objectives	IOS LWD W-PLAN PH 10x, 20x, 40x, anti-fungus treated
Stage	Fixed, 250×160 mm, glass and metal round inserts
Condenser	Abbe, N.A. 0.30, LWD 72 mm extendable up to 135 mm, phase contrast slider
Transmitted illumination	X-LED8, 8 W, manual brightness control
Fluorescence attachment	3 positions, blue and green filter sets included
Blue filter set (included)	Excitation 465-495 nm, dichroic 505 nm, emission 515LP nm
Green filter set (included)	Excitation 527-553 nm, dichroic 565 nm, emission 575LP nm
Fluorescence light source	HBO 100 W high pressure mercury bulb, 300 hours lifetime
Fluorescence power supply	External, 100-240 Vac 50/60 Hz, output 130 W
Product weight	14 kg

Options and Accessories – IM-300F

The IM-300F ships with IOS LWD W-PLAN PH 10x, 20x, and 40x objectives and blue and green fluorescence filter sets as standard. Additional accessories extend the system.

Mechanical stage, 250×230 mm, X-Y translation range 120×80 mm (optional)
Metallic interchangeable inserts for slides, Petri dishes, Terasaki, and multi-well plates (optional)
Holder for 2 slides (optional)
Holder for Utermohl chamber (optional)