Optika IM-300FL4 Inverted HBO fluorescence microscope, 400x, IOS LWD PLAN, B&G filter set

€10.768,00 excl. VAT

Estimated delivery time: 5-7 weeks

The Optika IM-300FL4 is an inverted trinocular HBO fluorescence microscope for live cell culture fluorescence work. It carries IOS LWD U-PLAN F objectives at 10x, 20x, and 40x, optimized for fluorescence rather than phase contrast, and the condenser extends its working distance up to 135 mm for tall culture vessels. The fluorescence attachment has 4 positions with blue and green filter sets included, plus room for UV and V filter sets on request. It suits life science laboratories that need dedicated fluorescence performance on an inverted stand, with wider filter capacity than the 3-position IM-300F.

Inverted HBO Fluorescence Microscope with a 4-Position Filter Attachment

The Optika IM-300FL4 brings dedicated fluorescence objectives and a 4-position filter attachment to an inverted stand for live cell culture work. Blue and green filter sets are included as standard, and UV and V filter sets can be added for broader fluorochrome coverage.

IOS LWD U-PLAN F objectives at 10x, 20x, and 40x, optimized for fluorescence
4-position fluorescence attachment, blue and green filter sets included
UV and V filter sets available on request for broader coverage
Condenser working distance extendable up to 135 mm for tall culture vessels
HBO 100 W high pressure mercury lamp for fluorescence excitation

Optika IM-300FL4 Inverted Trinocular HBO Fluorescence Microscope

The Optika IM-300FL4 is an inverted trinocular microscope from the IM-300 series, built for dedicated fluorescence work with a 4-position filter attachment.

IOS LWD U-PLAN F objectives are optimized for fluorescence detection, with an extendable working distance condenser for tall culture vessels.

4-Position Fluorescence Attachment

The fluorescence attachment ships with blue and green filter sets installed, and accepts UV and V filter sets on request, giving broader fluorochrome coverage than a 3-position holder.

IOS LWD U-PLAN F Objectives for Fluorescence

The objective set covers 10x, 20x, and 40x, optimized for fluorescence work rather than phase contrast, infinity corrected and anti-fungus treated with a 45 mm parfocal distance.

Extendable Long Working Distance Condenser

The Abbe condenser, N.A. 0.30, has a standard 72 mm working distance that extends up to 135 mm, with a color filter slider for the transmitted light path.

HBO Mercury Lamp Fluorescence Source

The fluorescence attachment is powered by a 100 W HBO high pressure mercury bulb with a 300 hour lifetime, with a manual filter set selection and centerable field diaphragm.

Ideal Applications

The IM-300FL4 suits laboratories that need dedicated fluorescence performance on an inverted stand.

Live cell culture fluorescence imaging
Immunofluorescence with blue, green, UV, and V fluorochromes
Multi-channel fluorescence research protocols
Research applications needing tall culture vessel compatibility
Cell biology laboratories with broader fluorochrome needs

Choose the IM-300FL4 when:

You need dedicated fluorescence objectives rather than phase contrast
You want a 4-position filter attachment for wider fluorochrome coverage
You need an extendable working distance condenser for tall vessels
You plan to add UV or V filter sets alongside blue and green

Optika Value Proposition

The Optika IM-300FL4 combines IOS LWD U-PLAN F fluorescence objectives with a 4-position HBO filter attachment, on an inverted trinocular stand with an extendable long working distance condenser.

Technical specifications – IM-300FL4

Specification	IM-300FL4
Type	Inverted, aluminum die-cast
Head	Trinocular, Siedentopf, split ratio 100/0 – 0/100, 45 degree inclination
Eyepieces	PL 10x, field number 22 mm, high eyepoint, retractable protections
Objectives	IOS LWD U-PLAN F 10x, 20x, 40x, anti-fungus treated
Stage	Fixed, 250×160 mm, glass and metal round inserts
Condenser	Abbe, N.A. 0.30, LWD 72 mm extendable up to 135 mm, color filter slider
Transmitted illumination	X-LED8, 8 W, manual brightness control
Fluorescence attachment	4 positions, blue and green filter sets included, UV and V optional
Blue filter set (included)	Excitation 465-495 nm, dichroic 505 nm, emission 515LP nm
Green filter set (included)	Excitation 527-553 nm, dichroic 565 nm, emission 575LP nm
Fluorescence light source	HBO 100 W high pressure mercury bulb, 300 hours lifetime
Fluorescence power supply	External, 100-240 Vac 50/60 Hz, output 130 W
Product weight	15 kg

Options and Accessories – IM-300FL4

The IM-300FL4 ships with IOS LWD U-PLAN F 10x, 20x, and 40x objectives and blue and green fluorescence filter sets as standard. Additional filter sets and accessories extend the system.

UV filter set, excitation 325-375 nm, dichroic 415 nm, emission 435LP nm (optional)
V filter set, excitation 390-420 nm, dichroic 440 nm, emission 450LP nm (optional)
Mechanical stage, 250×230 mm, X-Y translation range 120×80 mm (optional)
Metallic interchangeable inserts for slides, Petri dishes, Terasaki, and multi-well plates (optional)