Optika B-510FL Trinocular HBO fluorescence microscope, 400x, IOS PLAN F, B&G filter set

€5.137,00 excl. VAT

Estimated delivery time: 5-7 weeks

The Optika B-510FL is a trinocular HBO fluorescence microscope built for research applications with fluorescence. It carries Semi-Apochromatic IOS W-PLAN F objectives from 4x to 40x, giving higher resolution and greater numerical apertures for enhanced contrast, including under UV filters. The 100 W HBO high pressure mercury lamp is centrable and ships with blue and green filter sets on a 4-position slider. Full Kohler illumination and a rackless double layer stage support sharp, repeatable observation. It suits research laboratories that need the widest fluorochrome compatibility, with a filter holder that accepts additional custom filter sets.

Trinocular HBO Fluorescence Microscope for Research-Grade Fluorochrome Detection

The Optika B-510FL is built for research applications with fluorescence, the most used and diffused method worldwide for this purpose. Semi-Apochromatic IOS W-PLAN F objectives give higher resolution and greater numerical apertures for enhanced contrast, and the 4-position filter slider supports additional custom filter sets beyond the blue and green sets included as standard.

100 W HBO high pressure mercury lamp, centrable, 300 hours lifetime
Semi-Apochromatic IOS W-PLAN F objectives from 4x to 40x for enhanced contrast
Blue and green filter sets included on a 4-position slider
Full Kohler illumination with a rackless double layer stage
Supports additional custom filter sets such as V and UV on request

Optika B-510FL Trinocular HBO Fluorescence Microscope

The Optika B-510FL is a trinocular HBO fluorescence microscope from the B-510 research series, built around the 100 W high pressure mercury lamp, the most used fluorescence method worldwide for research applications.

Semi-Apochromatic IOS W-PLAN F objectives pair with full Kohler illumination to give sharp, contrasted images, including when detecting fluorescence under UV filters.

Semi-Apochromatic IOS W-PLAN F Objectives

The objective set covers 4x, 10x, 20x, and 40x, with superior resolution and greater numerical apertures for enhanced contrast, and performs well for fluorescence detection when using a UV filter.

HBO Mercury Lamp with 4-Position Filter Slider

The 100 W HBO high pressure mercury lamp is centrable with a 300 hour lifetime. Blue and green filter sets are included as standard on the 4-position slider, which also accepts additional custom filter sets such as V and UV, for fluorochromes including ANS, Fluorescamine, Catecholamine, AMCA, DAPI, Hoechst, and Indo-1.

Full Kohler Illumination and Swing-out Condenser

X-LED3 transmitted illumination with full Kohler adjustment works with a swing-out condenser, N.A. 0.2/0.9, delivering contrasted and sharp images across the brightfield light path.

Rackless Double Layer Stage

The 233×147 mm double layer mechanical stage moves on a rackless, belt-driven mechanism with a 78×54 mm X-Y moving range and a double vernier scale, right-handed for ergonomic positioning.

Ideal Applications

The B-510FL suits research laboratories that need the widest fluorochrome compatibility.

Life science and biomedical fluorescence research
Immunofluorescence with custom filter sets
Fluorochrome detection under UV excitation
Materials science fluorescence applications
Research applications needing a 4-position filter slider

Choose the B-510FL when:

You need traditional HBO mercury lamp fluorescence for research
You want Semi-Apochromatic objectives for enhanced contrast
You need a 4-position filter slider for custom filter sets
You need brightfield and fluorescence on a research-grade trinocular stand

Optika Value Proposition

The Optika B-510FL combines Semi-Apochromatic IOS W-PLAN F optics with HBO mercury lamp fluorescence and full Kohler illumination on a trinocular stand, backed by Optika 10-year mechanical and optical warranty plus lifetime X-LED warranty on the transmitted light source.

Technical specifications – B-510FL

Specification	B-510FL
Type	Upright, aluminum die-cast, transportation handle
Head	Trinocular, Siedentopf, split ratio 100/0 – 50/50 – 0/100, 30 degree inclination
Eyepieces	PL 10x, field number 22 mm, high eyepoint, retractable protections
Objectives	IOS W-PLAN F Semi-Apochromatic 4x, 10x, 20x, 40x, anti-fungus treated
Stage	Double layer, 233×147 mm, rackless, moving range 78×54 mm
Condenser	Swing-out, N.A. 0.2/0.9, iris diaphragm, centerable
Transmitted illumination	X-LED3, 3.6 W, manual brightness control, full Kohler illumination
Fluorescence light source	HBO 100 W high pressure mercury bulb, 300 hours lifetime
Fluorescence filter positions	4-position slider, manual selection, shutter
Blue filter set (included)	Excitation 460-495 nm, dichroic 505 nm, emission 510LP nm
Green filter set (included)	Excitation 510-550 nm, dichroic 570 nm, emission 575LP nm
Fluorescence power supply	External, 100-240 Vac 50/60 Hz, output 130 W
Product weight	14.2 kg

Options and Accessories – B-510FL

The B-510FL ships with IOS W-PLAN F objectives and blue and green fluorescence filter sets as standard, with one HBO bulb included. Additional filter sets and accessories extend the system.

UV filter set, excitation 325-375 nm, dichroic 415 nm, emission 435LP nm (optional)
V filter set, excitation 390-420 nm, dichroic 440 nm, emission 455LP nm (optional)
M-635 heating stage with digital temperature controller
M-069 external rechargeable battery pack
15104 cleaning kit