Optika B-383FL Trinocular HBO Fluorescence Microscope

The Optika B-383FL is a trinocular fluorescence microscope from the B-380 series, built around a traditional HBO 100 W high pressure mercury bulb, the most used fluorescence method worldwide for research purposes.

IOS N-PLAN brightfield objectives from 4x to 100x oil share the stand with the epi-fluorescence attachment, so users move between brightfield and fluorescence observation on one microscope.

HBO Mercury Lamp Fluorescence

The HBO 100 W high pressure mercury bulb gives an outstanding flexibility of use, and the blue and green filter sets supplied as standard allow the visualization of fluorochromes such as Auramine O, Acridine Yellow, FITC, and GFP (S65T) with the blue filter set, plus Rhodamine, Nile Red, Cy5.5, and Propidium Iodide with the green filter set.

2-Position Filter Holder for Future Upgrades

The filter holder accepts additional specific filter sets beyond the blue and green sets included as standard, so the system can be upgraded as fluorochrome needs change.

IOS N-PLAN Objectives for Brightfield

The objective set covers 4x, 10x, 40x, and 100x oil/water immersion, plan achromatic corrected with a DIN 45 parfocal distance and anti-fungus treatment.

Trinocular Head for Documentation

The trinocular head adds a camera port for photographic or digital documentation of fluorescence images alongside visual observation.

Ideal Applications

The B-383FL suits laboratories that need traditional HBO fluorescence with brightfield capability.

• Life science and biomedical research fluorescence work
• Immunofluorescence and fluorochrome-based staining
• Materials science fluorescence applications
• Combined brightfield and fluorescence workflows
• Camera-based fluorescence image documentation

Choose the B-383FL when:

• You need traditional HBO mercury lamp fluorescence for research use
• You want blue and green filter sets included as standard
• You need flexibility to add other filter sets later
• You need brightfield and fluorescence on the same trinocular stand

Optika Value Proposition

The Optika B-383FL combines HBO mercury lamp fluorescence with IOS N-PLAN brightfield optics on a trinocular stand, backed by Optika 10-year mechanical and optical warranty plus lifetime X-LED warranty on the transmitted light source.